RT-PCR Protocols - Methods in Molecular Biology
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چکیده
منابع مشابه
Methods in Molecular Biology PCR Protocols SECOND EDITION
1. Introduction The polymerase chain reaction (PCR) is a powerful method for fast in vitro enzymatic amplifications of specific DNA sequences. PCR amplifications can be grouped into three different categories: standard PCR, long PCR, and multiplex PCR. Standard PCR involves amplification of a single DNA sequence that is less than 5 kb in length and is useful for a variety of applications, such ...
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For almost 30 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readilyreproducible step-by-step fashion, opening with a...
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Dengue is the most important arboviral disease transmitted to humans. In our laboratory, we have been working on the standardization of the polymerase chain reaction (PCR) diagnosis of this disease. In this work, we compared five commercial kits regularly used on reverse-transcription polymerase chain reaction (RT-PCR) protocols: two Two-Step kits (SuperScript II RT/Super Mix kit and reverse tr...
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ژورنال
عنوان ژورنال: European Journal of Histochemistry
سال: 2011
ISSN: 2038-8306,1121-760X
DOI: 10.4081/ejh.2011.br2